你正在启动的TK和启动plt的TK之间出现了gui-main循环冲突。如果你想在自己的gui中使用matplotlib，你 * 必须 * 自己嵌入它，而且你不能导入pyplot。所有让pyplot界面美妙的幕后魔术都是让你在这里一团糟的东西。
有关教程请参见here，有关mpl如何嵌入请参见here。
另见：

• 无法保存matplotlib. figure图形，画布为"无"
• 关闭控制台时出现Python Matplotlib运行时错误
• Matplotlib条形图在一个wx框架而不是一个新窗口中

我现在通过使用matplotlib tk后端分别绘制每个窗口来解决这个问题。显然PyPlot干扰了tkinter根窗口。

import numpy as np
import matplotlib
matplotlib.use('TkAgg')

from numpy import arange, sin, pi
from matplotlib.backends.backend_tkagg import FigureCanvasTkAgg, NavigationToolbar2TkAgg
from matplotlib.figure import Figure
import tkinter as tk
from tkinter import ttk
import queue, threading, time

def center_window(window_parent, w=300, h=20):
    # get screen width and height
    ws = window_parent.winfo_screenwidth()
    hs = window_parent.winfo_screenheight()
    # calculate position x, y
    x = (ws/2) - (w/2)
    y = (hs/2) - (h/2)
    window_parent.geometry('%dx%d+%d+%d' % (w, h, x, y))

def MAIN():
    PB = q.get()
    for i in np.arange(10):
        time.sleep(0.2)
        print(i)
        PB.step(10)
        PB.update()
    print("Done")

root = tk.Tk()
root.wm_title("Embedding in TK")

f = Figure(figsize=(5,4), dpi=100)
a = f.add_subplot(111)
t = arange(0.0,3.0,0.01)
s = sin(2*pi*t)

a.plot(t,s)
a.set_title('Tk embedding')
a.set_xlabel('X axis label')
a.set_ylabel('Y label')

#a tk.DrawingArea
root2 = tk.Tk()

PB = ttk.Progressbar(root2, orient = "horizontal",length=300, mode = 'determinate')
PB.pack()

canvas = FigureCanvasTkAgg(f, master=root)
canvas.show()
canvas.get_tk_widget().pack(side=tk.TOP, fill=tk.BOTH, expand=1)

toolbar = NavigationToolbar2TkAgg( canvas, root )
toolbar.update()
canvas._tkcanvas.pack(side=tk.TOP, fill=tk.BOTH, expand=1)

root2.iconify()
root2.update()
root2.deiconify()

center_window(root2)

q = queue.Queue()
q.put(PB)

T = threading.Thread(target=MAIN(), name="MAIN")
T.start()
T.join()

root2.quit()
root2.destroy()

tk.mainloop()

此答案作为CC BY-SA 3.0下OP throwaway17434打开matplotlib窗口时关闭tkinter进度条窗口问题的edit发布。