yamllint命令行工具可以完成您想要的任务：

sudo pip install yamllint

具体地说，它有一个规则key-duplicates，可以检测重复和相互覆盖的密钥：

$ yamllint test.yaml
test.yaml
  1:1       warning  missing document start "---"  (document-start)
  10:5      error    duplication of key "subkey5" in mapping  (key-duplicates)

(It有许多其他的规则，您可以启用/禁用或调整。）

覆盖内置加载器是一种更轻量级的方法：

import yaml
 # special loader with duplicate key checking
 class UniqueKeyLoader(yaml.SafeLoader):
     def construct_mapping(self, node, deep=False):
         mapping = []
         for key_node, value_node in node.value:
             key = self.construct_object(key_node, deep=deep)
             assert key not in mapping
             mapping.append(key)
         return super().construct_mapping(node, deep)

然后：

yaml_text = open(filename), 'r').read()
 data[f] = yaml.load(yaml_text, Loader=UniqueKeyLoader)

ErichBSchulz非常努力。谢谢你的固定代码。我在这里做了一些小改动。使用行和列更新文件名。

class UniqueKeyLoader(yaml.SafeLoader):
    def construct_mapping(self, node, deep=False):
        mapping = set()
        for key_node, value_node in node.value:
            each_key = self.construct_object(key_node, deep=deep)
        if each_key in mapping:
            raise ValueError(f"Duplicate Key: {each_key!r} is found in YAML File.\n"
                             f"Error File location: {key_node.end_mark}")
        mapping.add(each_key)
        return super().construct_mapping(node, deep)

with open(test_suite_full_path, 'r') as f:
    yaml_ret_dict = yaml.load(f, Loader=UniqueKeyLoader)