我开始使用python工具来合并文件：

with open('transcriptome.fa','wb') as transcriptome_file:
        for fasta_file in ['cDNA.fa','ncRNA.fa']:
            with open(fasta_file,'rb') as current_fasta:
                shutil.copyfileobj(current_fasta, transcriptome_file)

字符串
以及下载较大的文件：

with requests.get('http://ftp.ensembl.org/pub/current_fasta/'+species+'/cdna/'+cDNA_file_name, stream=True) as cDNA_request:
        with open('cDNA.fa.gz', 'wb') as cDNA_gz_file:
            shutil.copyfileobj(cDNA_request.raw, cDNA_gz_file)

型
尽管如此，我仍然需要运行一个外部程序blast，所以我使用subprocess.run和creationflag = subprocess.CREATE_NO_WINDOW参数如下：

if os.name == 'nt':
    blast_db_run = subprocess.run(['makeblastdb',
                            '-in', fasta_file,
                            '-dbtype', 'nucl',
                            '-out','blast_db'],
                            capture_output=True, 
                            creationflags = subprocess.CREATE_NO_WINDOW)

型
使用if语句，因为creationflags显然在非windows环境中不起作用。