我知道，我们可以创建Seurat对象从细胞游侠输出文件（条形码，功能，矩阵）。但我有一个csv格式的GSE单细胞数据集。我该如何用它来创建修拉对象呢？
谢谢.a = read.csv(<csv file >, header = TRUE)b = t(a)c = CreateSeuratObject(counts = b, project = "my_single_cell", min.cells = 3, min.features = 200)Error in CreateAssayObject(counts = counts, min.cells = min.cells, min.features = min.features, :
输入矩阵中不存在单元格名称（列名）dput(a[1:10, 1:10])
2.0293824672699, 0, 0, 1.67916893959045, 1.25670552253723, 0,
0, 2.35019993782043, 0), Lypla1 = c(0, 0, 0, 0, 0, 0, 0, 0, 0,
0), Gm37988 = c(0, 0, 0, 0, 0, 0, 0, 0, 0, 0), Tcea1 = c(0, 0,
0, 0, 0, 0, 0, 0, 0, 0), Atp6v1h = c(0, 0, 0, 1.69750428199768,
0, 1.25670552253723, 0, 0, 0, 0), Rb1cc1 = c(0, 0, 0, 0, 0, 0,
0, 0, 0, 0), X4732440D04Rik = c(0, 0, 0, 0, 0, 0, 0, 0, 0, 0),
Pcmtd1 = c(0, 0, 0, 0, 0, 1.25670552253723, 0, 2.10469627380371,
0, 0), Gm26901 = c(0, 0, 0, 0, 0, 0, 0, 0, 0, 0)), row.names = c("AAACCCATCTGGAAGG-WT1",
"AAACGAACAACTCGTA-WT1", "AAACGAACACAGACGA-WT1", "AAACGAATCACGAACT-WT1",
"AAACGAATCAGGGTAG-WT1", "AAACGCTAGCTGACTT-WT1", "AAACGCTTCGACCTAA-WT1",
"AAAGAACAGAATCCCT-WT1", "AAAGTCCAGAGTGACC-WT1", "AAAGTCCAGTAAACAC-WT1"
), class = "data.frame")```
>
1条答案
按热度按时间idfiyjo81#
原始表格似乎行中有细胞，列中有基因，第一列中有细胞名称。要获得正确的行名称，请尝试以下操作：
编辑：该表似乎包含标准化的表达式值，而不是原始计数。修拉可能不是处理这种数据的合适工具。